Apta Hot-start Taq DNA polymerase - Parstous Biotechnology

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Apta Hot-start Taq DNA polymerase

PCR and RT Reagents > Hot Start DNA poly

Description:
This DNA polymerase is a mixture of Taq DNA polymerase and a temperature sensitive, aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 40°C, but releases the enzyme during normal PCR cycling conditions. The aptamer-based hot start mechanism does not require a separate high temperature incubation step to activate the enzyme. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers and providing higher specificity of DNA amplification.


Advantage:

Reduction of primer dimers
No inactivation time

Avoid non-specific bands

More stable than antibody based Hot-start taqs
More economical than antibody based Hot-start taqs



Applications:

Methylation detection PCR
Conventional PCR
T/A cloning

Amplification of a 125 bp fragment from CYP2C93 using taq DNA polymerase, hot-start taq DNA polymerase and Apta taq DNA polymerase. All the reactions were subjected to a pretreatment at 40˚C or 45˚C for 10 minutes before initial denaturation step at 94˚C to give them appropriate time and temperature to generate primer dimers. Apta-hot start taq has shown no primer dimers at 40˚C or 45˚C, the same as antibody-based hot-start taq at 45˚C. The colors in the photo have been inverted.

Cat No.

Size

Price (Rial)

C101011

250 U

1,700,000

C101012

500 U

3,200,000

 

Instructional Manual

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