The enhanced chemiluminescence detection reagent is recommended for use in horseradish peroxidase (HRP)-based Western Blotting procedures. Provided as a two-component system, this kit contains Solution A and Solution B. In the presence of peroxidase, the reagent emits chemiluminescent light. The chemiluminescent light emitting can be quantitatively detected via regular autoradiograph film, CCD camera, or chemiluminescence reading device. In immunoblotting procedure, this reagent kit offers a clean detection performance on PVDF and nitrocellulose membrane blots.