This kit has been designed for the detection of HLA B27 alleles (*2701 - *2735), based on the allele-specific primers. A fragment of human housekeeping gene is also amplifies as PCR internal control. Each kit offers Pars Tous’s optimal primer buffer system which will enhance amplification specificity with a detection limit of 10 ng per reaction. PCR amplification kit includes all necessary PCR amplification reagents with an exception of mineral oil.
The HLA B27 render a band of approximately 140 bp and internal control primers amplify a 430 bp fragment.