KlenTaq DNA polymerase - Parstous Biotechnology

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KlenTaq DNA polymerase

PCR and RT Reagents > DNA polymerase enzymes

KlenTaq DNA Polymerase is missing the N-terminal portion of the gene, encoding Thermus aquaticus (Taq) DNA polymerase, leaving a highly active and even more thermal stable DNA polymerase activity. This enzyme keeps significant activity after exposure to 99°C or repeated exposure to 98°C. The missing N-terminal portion is homologous to the 5’
3’ exonuclease region of E. coli. DNA polymerase I. KlenTaq is therefore similar to, yet distinct from, Hoffman LaRoche’s stoffel Fragment.


Wide range of optimal MgCl2 cocentartion: The optimal range of Mg 2+ concentration for KlenTaq is broader than for the majority of thermostable polymerases.
Lower error rate: The mutation rate during polymerization is twofold lower for KlenTaq in comparison with full-length Taq DNA polymerase.
Suitable for mutation analysis with mutation-specific oligonucleotides: KlenTaq has a very low background ability to extend a mismatched 3’-oligonucleotide end making it suitable for mutation analysis with mutation-specific oligonucleotides.
TA cloning: Amplicons are T/A Cloning compatible

Klentaq DNA polymerase amplifies DNA fragments in a wide range of MgCl2 without nonspecific bands, however, optimal MgCl2 concentration is near 3.5 mM.

Cat No.


Price (Rial)


250 U



500 U



Instructional Manual

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