KlenTaq DNA polymerase - Parstous Biotechnology

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KlenTaq DNA polymerase

PCR and RT Reagents > DNA polymerase enzymes

Description:
KlenTaq DNA Polymerase is missing the N-terminal portion of the gene, encoding Thermus aquaticus (Taq) DNA polymerase, leaving a highly active and even more thermal stable DNA polymerase activity. This enzyme keeps significant activity after exposure to 99°C or repeated exposure to 98°C. The missing N-terminal portion is homologous to the 5’
3’ exonuclease region of E. coli. DNA polymerase I. KlenTaq is therefore similar to, yet distinct from, Hoffman LaRoche’s stoffel Fragment.

Adavatages:

Wide range of optimal MgCl2 cocentartion: The optimal range of Mg 2+ concentration for KlenTaq is broader than for the majority of thermostable polymerases.
Lower error rate: The mutation rate during polymerization is twofold lower for KlenTaq in comparison with full-length Taq DNA polymerase.
Suitable for mutation analysis with mutation-specific oligonucleotides: KlenTaq has a very low background ability to extend a mismatched 3’-oligonucleotide end making it suitable for mutation analysis with mutation-specific oligonucleotides.
TA cloning: Amplicons are T/A Cloning compatible

Klentaq DNA polymerase amplifies DNA fragments in a wide range of MgCl2 without nonspecific bands, however, optimal MgCl2 concentration is near 3.5 mM.


Cat No.

Size

Price (Rial)

C101121

250 U

750,000

C101122

500 U

1,400,000

 

Instructional Manual

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