The Low Molecular Weight Protein Marker for SDS electrophoresis is a liquid mixture of six purified proteins ranging from 14000 to 96000 Dalton when used in denaturing polyacrylamide, for use in molecular weight estimation in the presence of the detergent sodium dodecyl sulphate (SDS).
Phosphorylase b: 50 μg, 97000 (Mr), rabbit muscle (source), Albumin: 50 μg, 66000 (Mr), bovine serum (source), Ovalbumin: 100 μg, 45000 (Mr), chicken egg white (source), Carbonic anhydrase: 40 μg, 30000 (Mr), bovine erythrocyte (source), Trypsin inhibitor: 37.5 μg, 20100 (Mr), soybean (source), Lysozyme: 50 μg, 14400 (Mr), egg white (source).
250 ul 278 ug 50 lanes
500 ul 575 ug 100 lanes
Fig 1. LMW protein marker stained with Coomassie Brilliant Blue. 5 μl of marker (10 μl of sample buffer diluted) were loaded on a 12.5% polyacrylamide gel. The gel was run at a constant current of 120 V for 1 hour, 55 minutes on a Bio-