Pfu DNA polymerase - Parstous Biotechnology

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Pfu DNA polymerase

PCR and RT Reagents > DNA polymerase enzymes

Description:

Pfu DNA Polymerase is a thermostable enzyme with a molecular weight of 90 kDa. It catalyzes the polymerization of nucleotides into duplex DNA in the 5'_3' direction, resulting in blunt-ended PCR products without 3'-dA overhangs.
Pfu DNA Polymerase exhibits 3'
_5' exonuclease (proofreading) activity that enables the polymerase to correct the mis-incorporation of nucleotide, and lacks 5'_3' exonuclease activity. It is suitable for PCR and primer extension reaction that requires high fidelity when the PCR fragment is relatively shorter than 3kb.

Adavatages:

Ultrapure recombinant enzyme.
The enzyme exhibits 3´>5´ proofreading activity, resulting in over 10-fold higher PCR fidelity than possible with Taq DNA Polymerases
The enhanced performance by new formula buffer.

Purification:

NI-IDA affinity chromatography

Stability:

Two years at -20 C

Source:

Pfu DNA Polymerase (recombinant) E.coli with a cloned pol gene from Pyrococcus furiosus.

Molecular weight:

Molecular Weight 90 kDa monomer.

Includes
Pfu DNA Polymerase 5 U/µL

 5X Pfu Buffer with (NH4)2SO4
 25 mM MgCl2



Analysis of Parstous Taq and Pfu DNA polymerase on 12.5%  polyacrylamide gel electrophoresis.

Cat No.

Size

Price (Rial)

C101141

250 U

1,400,000

C101142

500 U

2,500,000

 

Instructional Manual

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