Thermo Resistant RT - Parstous Biotechnology

Go to content

Main menu

Thermo Resistant RT

PCR and RT Reagents > cDNA synthesis reagents

Description:
This a genetically modified RNA-dependent DNA polymerase requiring a DNA primer and an RNA template to synthesize a complementary DNA strand. Thermo-resistant H Minus M-MuLV Reverse Transcriptase has no RNase H activity. Therefore, degradation of RNA does not occur during first strand cDNA synthesis, resulting in higher yields of full-length cDNA from long templates compare to other reverse transcriptases. The enzyme maintains activity over a wide temperature range (42-52°C) which makes it an ideal tool for reverse transcription of RNAs having a high degree of secondary structure.


Advantages:

Optimal activity at 47-48°C
Reverse transcription of RNAs with a high degree of secondary structure.
No RNase H activity

More stable than Wild type MMuLV


Applications:

Single strand cDNA synthesis for PCR or Q-PCR

 

Cat No.

Size

Price (Rial)

C101131

100 ul

2,800,000

 

Instructional Manual

Back to content | Back to main menu